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Cancer Gastric Chemoprevention: Isolation of Gastric Tumor-Initiating Cells

  • Federica MoriEmail author
  • Valeria Canu
  • Laura Lorenzon
  • Alfredo Garofalo
  • Giovanni Blandino
  • Sabrina Strano
Part of the Methods in Molecular Biology book series (MIMB, volume 1379)

Abstract

Gastric cancer is an important healthcare problem and represents the second leading cause of death for malignant disease worldwide. In the Western world, the diagnosis is done at late stage when treatments can be only palliative. Searches for new therapeutic regimens as well as for new biomarkers are in progress.

To reduce cancer mortality is crucial the prevention of the lesion at earlier stages. Therefore, new bullets to prevention are needed.

Nowadays, studies relating to different kinds of tumor are unanimous in considering cancer stem cells (CSCs) as “the core” of the tumor and the responsible of tumor chemoresistance and relapse.

This chapter aims to provide the instructions to (1) isolate, (2) grow, and (3) validate, both in vivo and in vitro, the gastric CSC subpopulation.

Key words

Gastric cancer Chemoprevention Cancer stem cells Patient-derived xenograft Chemoresistance 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Federica Mori
    • 1
    Email author
  • Valeria Canu
    • 2
  • Laura Lorenzon
    • 3
  • Alfredo Garofalo
    • 4
  • Giovanni Blandino
    • 5
  • Sabrina Strano
    • 6
    • 7
  1. 1.Molecular Chemoprevention Unit, Molecular Medicine DepartmentRegina Elena National Cancer InstituteRomeItaly
  2. 2.Translational Oncogenomic Unit, Molecular Medicine DepartmentRegina Elena National Cancer InstituteRomeItaly
  3. 3.Surgical and Medical Department of Translational Medicine, Faculty of Medicine and Psychology, Sant’Andrea HospitalUniversity of Rome “La Sapienza”RomeItaly
  4. 4.Department of GI SurgeryRegina Elena National Cancer InstituteRomeItaly
  5. 5.Laboratory of Translational Oncogenomics, Molecular Medicine DepartmentRegina Elena National Cancer InstituteRomeItaly
  6. 6.Molecular Cancer Chemoprevention Unit, Molecular Medicine DepartmentRegina Elena National Cancer InstituteRomeItaly
  7. 7.Department of OncologyJuravinski Cancer Center-McMaster University HamiltonHamiltonItaly

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