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DNA Extraction from Seeds

  • Protocol
Sample Preparation Techniques for Soil, Plant, and Animal Samples

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

The extraction of DNA from seeds is a very important step in every molecular study involving plant genetics. Seeds possesses many compounds that effectively interfere with DNA extraction and subsequently with downstream procedures. Despite the importance of this subject, little attention is given to improving and standardizing the current protocols. Thus, each study has to adapt and develop the best protocol modifications adjusted to its own specific requirements. After discussing several biotechnical difficulties and different solutions for each one, we propose a widely used protocol and diverse possibilities of protocol changes displayed in a diagram to ease every possible change and adjustment.

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Author information

Authors and Affiliations

  1. Evolutionary Genetics and Molecular Biology Department, PPGGEV, Universidade Federal de São Carlos—UFSCar, Rodovia Washington Luiz Km 235, São Carlos, SP, 13560-970, Brazil

    Célio Dias Santos Júnior B.S.

  2. Genetic and Biochemistry Institute, Federal University of Uberlândia, Avenida Pará, Uberlândia, MG, 38400-902–CP 592, Brazil

    Natália Melquie Monteiro Teles B.S.

  3. Institue of Genetic and Biochemistry—INGEB, Universidade Federal de Uberlândia, Avenida Pará, Uberlândia, MG, 38400-902–CP 592, Brazil

    Denis Prudêncio Luiz M.S.

  4. Institute of Tropical Pathology and Public Health (IPTSP), Federal University of Goias- UFG, Street 235, Setor Universitário, Goiânia, GO, CEP 74605-050, Brazil

    Thais Ferreira Isabel Ph.D.

Authors
  1. Célio Dias Santos Júnior B.S.
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  2. Natália Melquie Monteiro Teles B.S.
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  3. Denis Prudêncio Luiz M.S.
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  4. Thais Ferreira Isabel Ph.D.
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Corresponding author

Correspondence to Célio Dias Santos Júnior B.S. .

Editor information

Editors and Affiliations

  1. Department of Engineering Design Technology, Cerritos College, Norwalk, California, USA

    Miodrag Micic

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Júnior, C.D.S., Teles, N.M.M., Luiz, D.P., Isabel, T.F. (2016). DNA Extraction from Seeds. In: Micic, M. (eds) Sample Preparation Techniques for Soil, Plant, and Animal Samples. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3185-9_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3185-9_18

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3184-2

  • Online ISBN: 978-1-4939-3185-9

  • eBook Packages: Springer Protocols

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