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Nucleic Acid Sample Preparation from Teeth/Dental Remains

  • Protocol
Sample Preparation Techniques for Soil, Plant, and Animal Samples

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

Teeth are a reliable source of DNA that have been used in archaeological, medical, and forensic studies to gain insight into the origins, migration, health, biogeographic ancestry, and identification of deceased individuals and populations. The unique structure of teeth provides enhanced protection to endogenous DNA from the combined effects of degradation and contamination but also introduces specific challenges to the sampling and extraction processes. In this chapter we review the structure and composition of teeth and discuss sampling and extraction processes to effectively utilize these tissues for subsequent genetic analyses.

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Author information

Authors and Affiliations

  1. Australian Centre for Ancient DNA, Environment Institute and School of Earth and Environmental Sciences, University of Adelaide, Adelaide, SA, 5005, Australia

    Denice Higgins B.D.S. G. Dip. For. Odont. & Jeremy J. Austin Ph.D.

  2. Sciences Department, Museum Victoria, Carlton Gardens, Melbourne, VIC, 3001, Australia

    Jeremy J. Austin Ph.D.

Authors
  1. Denice Higgins B.D.S. G. Dip. For. Odont.
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  2. Jeremy J. Austin Ph.D.
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Corresponding author

Correspondence to Denice Higgins B.D.S. G. Dip. For. Odont. .

Editor information

Editors and Affiliations

  1. Department of Engineering Design Technology, Cerritos College, Norwalk, California, USA

    Miodrag Micic

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Higgins, D., Austin, J.J. (2016). Nucleic Acid Sample Preparation from Teeth/Dental Remains. In: Micic, M. (eds) Sample Preparation Techniques for Soil, Plant, and Animal Samples. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3185-9_13

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  • DOI: https://doi.org/10.1007/978-1-4939-3185-9_13

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3184-2

  • Online ISBN: 978-1-4939-3185-9

  • eBook Packages: Springer Protocols

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