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TAG Sequence Identification of Genomic Regions Using TAGdb

  • Pradeep RuperaoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1374)

Abstract

Second-generation sequencing (SGS) technology has enabled the sequencing of genomes and identification of genes. However, large complex plant genomes remain particularly difficult for de novo assembly. Access to the vast quantity of raw sequence data may facilitate discoveries; however the volume of this data makes access difficult. This chapter discusses the Web-based tool TAGdb that enables researchers to identify paired read second-generation DNA sequence data that share identity with a submitted query sequence. The identified reads can be used for PCR amplification of genomic regions to identify genes and promoters without the need for genome assembly.

Key words

Second-generation sequencing Orthologous sequences Illumina Promoter discovery 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.School of Agriculture and Food SciencesUniversity of QueenslandSt. LuciaAustralia
  2. 2.School of Plant BiologyUniversity of Western AustraliaPerthAustralia

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