Abstract
Fission yeast cells can be synchronized by cell cycle arrest and release or by size selection. Cell cycle arrest synchronization is based on the block and release of temperature-sensitive cell cycle mutants or treatment with drugs. The most widely used approaches are cdc10-129 for G1; hydroxyurea (HU) for early S-phase; cdc25-22 for G2, and nda3-KM311 for mitosis. Cells can also be synchronized by size selection using centrifugal elutriation or a lactose gradient. Here we describe the methods most commonly used to synchronize fission yeast cells.
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Acknowledgements
We thank N. Skinner for corrections to the manuscript. This work was funded by grants BFU2011-28274 from the Spanish Ministry of Economy and Competitiveness MINECO and CSI151U13 from la Junta de Castilla y León. M.T.-P. is a recipient of a CSIC JAE-predoctoral fellowship.
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Tormos-Pérez, M., Pérez-Hidalgo, L., Moreno, S. (2016). Fission Yeast Cell Cycle Synchronization Methods. In: Sanchez-Diaz, A., Perez, P. (eds) Yeast Cytokinesis. Methods in Molecular Biology, vol 1369. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3145-3_20
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DOI: https://doi.org/10.1007/978-1-4939-3145-3_20
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3144-6
Online ISBN: 978-1-4939-3145-3
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