Protocol

Macro-Glycoligands

Volume 1367 of the series Methods in Molecular Biology pp 195-206

Oriented Immobilized Sialyloligo-macroligand Microarray

  • Satya Nandana NarlaAffiliated withDepartment of Chemistry, Chemical and Biomedical Engineering, Center for Gene Regulation in Health and Disease (GRHD), Cleveland State University
  • , Xue-Long SunAffiliated withDepartment of Chemistry, Chemical and Biomedical Engineering, Center for Gene Regulation in Health and Disease (GRHD), Cleveland State University Email author 

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Abstract

Silaic acid is the most common terminal glycan on cell surface glycoproteins and glycolipids, involving in many biological processes. Studying interactions between multivalent sialic acid scaffolds and proteins binding to it will give incredible information in understanding the biological process the sialic acid is involved in. Here we describe chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications.

Key words

Sialic acid Glycan array Glycopolymer Lectin Hemagglutinin SPR