Oriented Immobilized Sialyloligo-macroligand Microarray

Part of the Methods in Molecular Biology book series (MIMB, volume 1367)

Abstract

Silaic acid is the most common terminal glycan on cell surface glycoproteins and glycolipids, involving in many biological processes. Studying interactions between multivalent sialic acid scaffolds and proteins binding to it will give incredible information in understanding the biological process the sialic acid is involved in. Here we describe chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications.

Key words

Sialic acid Glycan array Glycopolymer Lectin Hemagglutinin SPR 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Chemistry, Chemical and Biomedical Engineering, Center for Gene Regulation in Health and Disease (GRHD)Cleveland State UniversityClevelandUSA

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