Abstract
Glycosylated noble metal nanoparticles are a useful tool for probing biological binding events due to their aggregation-induced color changes, particularly for lectins that have multiple binding sites. To overcome the challenges of colloidal instability, which leads to false-positive results, it is essential to add polymeric coatings to these particles. Here we describe a versatile, and reliable, approach to enable coating of gold nanoparticles using well-defined polymers, with carbohydrate end groups. This produces multivalent nanoparticles that are both colloidally stable, but still retain their rapid colorimetric responses to lectin binding.
Key words
- Gold nanoparticle s
- Glycopolymers
- RAFT polymerization
- Lectins
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Acknowledgements
Equipment used was supported by the Innovative Uses for Advanced Materials in the Modern World (AM2), with support from Advantage West Midlands (AWM) and part funded by the European Regional Development Fund (ERDF). MIG was a Birmingham Science City Interdisciplinary Research Fellow funded by the Higher Education Funding Council for England (HEFCE). SJR acknowledges the EPSRC funded MOAC doctoral training centre for a studentship. CIB acknowledges the BBSRC for a studentship.
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Richards, SJ., Biggs, C.I., Gibson, M.I. (2016). Multivalent Glycopolymer-Coated Gold Nanoparticles. In: Sun, XL. (eds) Macro-Glycoligands. Methods in Molecular Biology, vol 1367. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3130-9_14
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DOI: https://doi.org/10.1007/978-1-4939-3130-9_14
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