Regulation of Activation Induced Deaminase (AID) by the hormone estrogen has important implications for understanding adaptive immune responses as well as the involvement of AID in autoimmune diseases and tumorigenesis. This chapter describes the general laboratory techniques for analyzing AID expression and activity induced by estrogen, focusing on the isolation and preparation of cells for hormone treatment and the subsequent analysis of AID responsiveness to estrogen at the RNA level and for determining the regulation of AID activity via estrogen by analyzing Ig switch circle transcripts and mutations in switch region loci.
Activation induced deaminase AID Estrogen Adaptive immune responses Splenic B-cells Ig switch circle transcripts Somatic hypermutations Autoimmune diseases
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S.P. was supported by FEBS long-term fellowship.
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