Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 Activation

  • Sara Clark
  • Kevin Pollard
  • Jennifer Rainville
  • Nandini Vasudevan
Part of the Methods in Molecular Biology book series (MIMB, volume 1366)


Phosphorylation of the serine residues in estrogen receptor (ER) α is important in transcriptional activation. Hence, methods to detect such posttranslational modification events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immuno-blotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estrogen receptor (mER), GPR30. Hence, phosphorylation represents a cross talk event between GPR30 and ERα and may be important in estrogen-regulated physiology.

Key words

Phosphorylation Serine Nuclear receptor Antibody Tissue Lysate Cell line GPR30 ERα 



This work was supported by funding supplied by the NSF CAREER IOS-1053716 to N.V. and a Louisiana Board of Regents Fellowship to K.P.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Sara Clark
    • 1
  • Kevin Pollard
    • 2
  • Jennifer Rainville
    • 1
  • Nandini Vasudevan
    • 1
    • 2
  1. 1.Department of Cell and Molecular BiologyTulane UniversityNew OrleansUSA
  2. 2.The Neuroscience ProgramTulane UniversityNew OrleansUSA

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