Abstract
Phosphorylation of the serine residues in estrogen receptor (ER) α is important in transcriptional activation. Hence, methods to detect such posttranslational modification events are valuable. We describe, in detail, the analysis of the phosphorylated ERα by electrophoretic separation of proteins and subsequent immuno-blotting techniques. In particular, phosphorylation of the ERα is one possible outcome of activation of the putative membrane estrogen receptor (mER), GPR30. Hence, phosphorylation represents a cross talk event between GPR30 and ERα and may be important in estrogen-regulated physiology.
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Acknowledgments
This work was supported by funding supplied by the NSF CAREER IOS-1053716 to N.V. and a Louisiana Board of Regents Fellowship to K.P.
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Clark, S., Pollard, K., Rainville, J., Vasudevan, N. (2016). Detection of the Phosphorylation of the Estrogen Receptor α as an Outcome of GPR30 Activation. In: Eyster, K.M. (eds) Estrogen Receptors. Methods in Molecular Biology, vol 1366. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3127-9_36
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DOI: https://doi.org/10.1007/978-1-4939-3127-9_36
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3126-2
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