Estrogen Receptor-α Knockout Mice

  • Per Antonson
  • Patricia Humire
  • Jan-Åke Gustafsson
Part of the Methods in Molecular Biology book series (MIMB, volume 1366)


Tissue specific knockout mice are valuable tools to study gene function in vivo. The method uses the Cre/loxP system in which loxP sites are cloned into the genome surrounding one or more exons of a gene and the targeted exon(s) are deleted when the Cre enzyme is expressed. Mouse lines that are prepared for the generation of knockout ERα mice have been developed independently by many research groups and the number of available transgenic mouse lines that express Cre under tissue specific promoters is large. Here, we describe how tissue specific ERα knockout mice are generated.

Key words

Estrogenreceptor Knockout mice Gene targeting Tissue specific Transgenic Cre enzyme Genotyping loxP Floxed 



This study was supported by a Grant from the Swedish Cancer Fund. J.-Å. G. is thankful to the Robert A. Welch Foundation for an endowment.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Per Antonson
    • 1
  • Patricia Humire
    • 1
  • Jan-Åke Gustafsson
    • 1
    • 2
  1. 1.Department of Biosciences and NutritionKarolinska InstitutetHuddingeSweden
  2. 2.Center for Nuclear Receptors and Cell Signaling, Department of Biology and BiochemistryUniversity of HoustonHoustonUSA

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