Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells

Part of the Methods in Molecular Biology book series (MIMB, volume 1366)

Abstract

Molecular signaling through both estrogen and microRNAs are critical for breast cancer development and growth. The activity of estrogen is mediated by transcription factors, the estrogen receptors. Here we describe a method for robust characterization of estrogen-regulated microRNA profiles. The method details how to prepare cells for optimal estrogen response, directions for estrogen treatment, RNA extraction, microRNA large-scale profiling, and subsequent confirmations.

Key words

Breast cancer MicroRNA Estrogen Estrogenreceptor Microarray qPCR 

Notes

Acknowledgements

This work was supported by NIH grant CA172437 and by co-funding from Marie Curie Actions FP7-PEOPLE-2011-COFUND (GROWTH 291795) via the VINNOVA programme Mobility for Growth.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Center for Nuclear Receptors and Cell Signaling, Department of Biology and BiochemistryUniversity of HoustonHoustonUSA
  2. 2.Department of Biology and BiochemistryCenter for Nuclear Receptors and Cell Signaling, University of HoustonHoustonUSA
  3. 3.SciLifeLab, Department of Proteomics and NanobiotechnologyKTH—Royal Institute of TechnologySolnaSweden

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