Estrogen Receptor Agonists and Antagonists in the Yeast Estrogen Bioassay

Part of the Methods in Molecular Biology book series (MIMB, volume 1366)

Abstract

Cell-based bioassays can be used to predict the eventual biological activity of a substance on a living organism. In vitro reporter gene bioassays are based on recombinant vertebrate cell lines or yeast strains and especially the latter are easy-to-handle, cheap, and fast. Moreover, yeast cells do not express estrogen, androgen, progesterone or glucocorticoid receptors, and are thus powerful tools in the development of specific reporter gene systems that are devoid of crosstalk from other hormone pathways. This chapter describes our experience with an in-house developed RIKILT yeast estrogen bioassay for testing estrogen receptor agonists and antagonists, focusing on the applicability of the latter.

Key words

Yeast estrogen bioassay In vitro Estrogenreceptor Estrogenantagonist 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Business Unit of Toxicology & Bioassays, RIKILT—Institute of Food SafetyWageningen University and Research CentreWageningenThe Netherlands

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