Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model
In vivo models to detect estrogenic compounds are very valuable for screening for endocrine disruptors. Here we describe the use of transgenic estrogen reporter zebrafish as an in vivo model for identification of estrogenic properties of compounds. Live imaging of these transgenic fish provides knowledge of estrogen receptor specificity of different ligands as well as dynamics of estrogen signaling. Coupled to image analysis, the model can provide quantitative dose-response information on estrogenic activity of chemical compounds.
Key wordsTransgeniczebrafish Estrogen Xenoestrogens Fluorescence microscopy Image analysis
The data presented here were generated in projects funded by grants from the Environmental Protection Agency (R834289), NIH (F32HD061119) and startup funds from Dept. of Pharmacology & Toxicology at the University of Alabama at Birmingham.
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