Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry
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Virus-Induced Gene Silencing (VIGS) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. In this chapter, we describe a high-throughput approach based on VIGS for the study of tomato fruit biochemistry. It comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the Tobacco Rattle Virus (TRV), and the agroinfiltration of tomato fruits mediated by Agrobacterium tumefaciens.
Key wordsVIGS Silencing Reverse genetics TRV Tomato
This work was supported by the Italian Ministry of Research (Project “Integrated Knowledge for the Sustainability and Innovation of Italian Agri-Food”), by the Italian Ministry of Agriculture (Projects “Nutrisol” and “Biomassval”), and by the European Commission (FP7 Project “From discovery to products: A next generation pipeline for the sustainable generation of high-value plant products,” contract no. 613513; Horizon 2020 project “Traditional tomato varieties and cultural practices: a case for agricultural diversification with impact on food security and health of European population,” contract no. 634561) and benefited from the networking activities of COST Action FA1106 “QualityFruit.” We thank Alessandro Nicolia for critical reading of the manuscript.
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