Plant Signal Transduction pp 133-144

Part of the Methods in Molecular Biology book series (MIMB, volume 1363) | Cite as

Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins

Abstract

Membrane proteins are difficult to study for numerous reasons. The surface of membrane proteins is relatively hydrophobic and sometimes very unstable, additionally requiring detergents for their extraction from the membrane. This leads to challenges at all levels, including expression, solubilization, purification, identification of associated proteins, and the identification of post-translational modifications. However, recent advances in immunoprecipitation technology allow to isolate membrane proteins efficiently, facilitating the study of protein-protein interactions, the identification of novel associated proteins, and to identify post-translational modifications, such as phosphorylation. Here, we describe an optimized immunoprecipitation protocol for plant plasma membrane receptor-like kinases.

Key words

Immunoprecipitation Protein phosphorylation Receptor-like kinase 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yasuhiro Kadota
    • 1
    • 2
  • Alberto P. Macho
    • 1
    • 3
  • Cyril Zipfel
    • 1
  1. 1.The Sainsbury LaboratoryNorwich Research ParkNorwichUK
  2. 2.Plant Immunity Research GroupRIKEN Center for Sustainable Resource ScienceYokohamaJapan
  3. 3.Shanghai Center for Plant Stress BiologyChinese Academy of SciencesShanghaiChina

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