Suppressor Screens in Arabidopsis
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Genetic screens have proven to be a useful tool in the dissection of biological processes in plants. Specifically, suppressor screens have been widely used to study signal transduction pathways. Here we provide a detailed protocol for ethyl methanesulfonate (EMS) mutagenesis used in our suppressor screens in Arabidopsis and discuss the basic principles behind suppressor screen design and downstream analyses.
Key wordsSuppressor screen EMSmutagenesis Arabidopsis
The authors thank the Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant Program, the Canadian Foundation of Innovation (CFI), and the University of British Columbia for supporting the various genetic screens we have conducted in the past decade. Ms. Kaeli Johnson is warmly thanked for critical reading of the manuscript.