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Studying the Translatome with Polysome Profiling

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Part of the Methods in Molecular Biology book series (MIMB,volume 1358)

Abstract

Polysome fractionation by sucrose density gradient centrifugation followed by analysis of RNA and protein is a technique that allows to understand the changes in translation of individual mRNAs as well as genome-wide effects on the translatome. Here, we describe the polysome profiling technique and RNA as well as protein isolation procedures from sucrose fractions.

Key words

  • Polysome
  • Profiling
  • Transcriptome
  • Translational control
  • Translatome
  • Sucrose gradient
  • RNA isolation
  • Protein isolation

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  • DOI: 10.1007/978-1-4939-3067-8_4
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Correspondence to Angelika Modelska .

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Zuccotti, P., Modelska, A. (2016). Studying the Translatome with Polysome Profiling. In: Dassi, E. (eds) Post-Transcriptional Gene Regulation. Methods in Molecular Biology, vol 1358. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3067-8_4

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  • DOI: https://doi.org/10.1007/978-1-4939-3067-8_4

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3066-1

  • Online ISBN: 978-1-4939-3067-8

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