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Visualizing mRNA Dynamics in Live Neurons and Brain Tissues

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Post-Transcriptional Gene Regulation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1358))

Abstract

Localization of mRNA plays a crucial role in a variety of neuronal processes including synaptogenesis, axonal guidance, and long-term plasticity. Recent advances in fluorescence imaging and RNA labeling techniques allow us to visualize how individual mRNA molecules are dynamically regulated inside live neurons and brain tissues. Here, we describe key methods in imaging mRNA dynamics, including preparation of neuron culture and brain slices from transgenic mice expressing GFP-labeled mRNA, high-resolution detection of single molecules, live tissue imaging, and analysis of mRNA transport.

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Acknowledgements

This work was supported by Research Resettlement Fund for the new faculty of Seoul National University to H.Y.P.

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Correspondence to Hye Yoon Park .

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Park, H.Y., Song, M. (2016). Visualizing mRNA Dynamics in Live Neurons and Brain Tissues. In: Dassi, E. (eds) Post-Transcriptional Gene Regulation. Methods in Molecular Biology, vol 1358. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3067-8_21

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  • DOI: https://doi.org/10.1007/978-1-4939-3067-8_21

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3066-1

  • Online ISBN: 978-1-4939-3067-8

  • eBook Packages: Springer Protocols

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