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Molecular Aspects of Conifer Zygotic and Somatic Embryo Development: A Review of Genome-Wide Approaches and Recent Insights

  • Jean-François Trontin
  • Krystyna Klimaszewska
  • Alexandre Morel
  • Catherine Hargreaves
  • Marie-Anne Lelu-Walter
Part of the Methods in Molecular Biology book series (MIMB, volume 1359)

Abstract

Genome-wide profiling (transcriptomics, proteomics, metabolomics) is providing unprecedented opportunities to unravel the complexity of coordinated gene expression during embryo development in trees, especially conifer species harboring “giga-genome.” This knowledge should be critical for the efficient delivery of improved varieties through seeds and/or somatic embryos in fluctuating markets and to cope with climate change. We reviewed “omics” as well as targeted gene expression studies during both somatic and zygotic embryo development in conifers and tentatively puzzled over the critical processes and genes involved at the specific developmental and transition stages. Current limitations to the interpretation of these large datasets are going to be lifted through the ongoing development of comprehensive genome resources in conifers. Nevertheless omics already confirmed that master regulators (e.g., transcription and epigenetic factors) play central roles. As in model angiosperms, the molecular regulation from early to late embryogenesis may mainly arise from spatiotemporal modulation of auxin-, gibberellin-, and abscisic acid-mediated responses. Omics also showed the potential for the development of tools to assess the progress of embryo development or to build genotype-independent, predictive models of embryogenesis-specific characteristics.

Key words

Developmental regulator Embryo patterning Gymnosperm Metabolome Proteome Somatic embryogenesis Transcriptome Stress 

Notes

Acknowledgements

The preparation of this chapter was supported through various projects funded by (1) the French National Research Agency (GENOQB: ANR-05-GPLA-027, SUSTAINPINE: ANR-09-KBBE-007, XYLOFOREST: ANR-10-EQPX-16), (2) the European Community’s Seventh Framework Programme (FP7/2007-2013, Grant Agreement n° 289841-PROCOGEN), and (3) the French Regional Councils of “Région Centre” (EMBRYOME: 33639, IMTEMPERIES: 2014-00094511) and “Région Aquitaine” (EMBRYO2011: 09012579-045). K.K. was supported by Natural Resources Canada, Canadian Forest Service. Mrs. Isabelle Lamarre (NRCan, CFS) is thanked for English editing.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Jean-François Trontin
    • 1
  • Krystyna Klimaszewska
    • 2
  • Alexandre Morel
    • 4
  • Catherine Hargreaves
    • 3
  • Marie-Anne Lelu-Walter
    • 4
  1. 1.FCBA, Pôle Biotechnologie et Sylviculture AvancéeCestasFrance
  2. 2.Natural Resources Canada, Canadian Forest ServiceLaurentian Forestry CentreStn. Sainte-FoyCanada
  3. 3.ScionRotoruaNew Zealand
  4. 4.INRA, UR 0588 Unité AméliorationGénétique et Physiologie ForestièresArdon, Orléans Cedex 2France

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