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In Vitro Embryogenesis in Higher Plants pp 431–438Cite as

Somatic Embryogenesis in Horse Chestnut (Aesculus hippocastanum L.)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1359))

Abstract

Embryogenic cultures of horse chestnut (Aesculus hippocastanum L.) can be obtained from different organs and tissues. We describe here the induction from stamen filaments and the procedures applied for the successive phases of somatic embryo development and maturation. Embryogenic tissues are obtained on Murashige and Skoog medium containing 9.0 μM 2,4-dichlorophenoxyacetic acid. Somatic embryos develop after transfer to hormone-free medium enriched with glutamine. Maturation and germination of isolated embryos are achieved by transfer to medium containing polyethylene glycol 4000 and activated charcoal, successive desiccation treatment, and cold storage at 4 °C for 8 weeks.

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Author information

Authors and Affiliations

  1. Istituto di Bioscienze e Biorisorse (IBBR) – CNR, sede Firenze, Via Madonna del Piano 10, Sesto Fiorentino, Firenze, Italy

    Maurizio Capuana

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  1. Maurizio Capuana
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Correspondence to Maurizio Capuana .

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Editors and Affiliations

  1. Dipartimento DEMETRA, Università degli Studi di Palermo, Palermo, Italy

    Maria Antonietta Germana

  2. National Research Council (CNR) IVALSA/Istituto per la Valorizzazione, Sesto Fiorentino, Florence, Italy

    Maurizio Lambardi

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Capuana, M. (2016). Somatic Embryogenesis in Horse Chestnut (Aesculus hippocastanum L.). In: Germana, M., Lambardi, M. (eds) In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3061-6_23

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  • DOI: https://doi.org/10.1007/978-1-4939-3061-6_23

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3060-9

  • Online ISBN: 978-1-4939-3061-6

  • eBook Packages: Springer Protocols

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