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Somatic Embryogenesis and Plant Regeneration of Brachiaria brizantha

  • Glaucia B. Cabral
  • Vera T. C. Carneiro
  • Diva M. A. Dusi
  • Adriana P. Martinelli
Part of the Methods in Molecular Biology book series (MIMB, volume 1359)

Abstract

The genus Brachiaria (Trin.) Griseb. belongs to the family Poaceae, order Poales, class Monocotyledonae. In Brachiaria brizantha (Hochst. ex A. Rich.) Stapf., embryogenic callus can be induced from seeds from apomictic plants, which results in high frequency somatic embryo development and plant regeneration. We report here a detailed protocol for callus induction from apomictic seed; followed by in vitro morphogenesis (somatic embryo and bud differentiation), plant regeneration, and acclimatization in the greenhouse. Important details regarding the positioning of seeds for callus induction and precautions to avoid endophytic contamination and the occurrence of albino plants are presented.

Key words

Albino plants Apomixis Caryopses Endophyte contamination Forage grass In vitro culture Monocot 

Notes

Acknowledgements

G.B.C. thanks Embrapa for a doctoral fellowship. A.P.M. acknowledges CNPq for a research fellowship (310.612/2011-0).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Glaucia B. Cabral
    • 1
  • Vera T. C. Carneiro
    • 1
  • Diva M. A. Dusi
    • 1
  • Adriana P. Martinelli
    • 2
  1. 1.Embrapa Genetic Resources and BiotechnologyParque Estação BiológicaBrasíliaBrazil
  2. 2.University of São Paulo, CENAPiracicabaBrazil

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