Somatic Embryogenesis in Olive (Olea europaea L. subsp. europaea var. sativa and var. sylvestris)

  • Eddo Rugini
  • Cristian Silvestri
Part of the Methods in Molecular Biology book series (MIMB, volume 1359)


Protocols for olive somatic embryogenesis from zygotic embryos and mature tissues have been described for both Olea europaea sub. europaea var. sativa and var. sylvestris. Immature zygotic embryos (no more than 75 days old), used after fruit collection or stored at 12–14 °C for 2–3 months, are the best responsive explants and very slightly genotype dependent, and one single protocol can be effective for a wide range of genotypes. On the contrary, protocols for mature zygotic embryos and for mature tissue of cultivars are often genotype specific, so that they may require many adjustments according to genotypes. The use of thidiazuron and cefotaxime seems to be an important trigger for induction phase particularly for tissues derived from cultivars. Up to now, however, the application of this technique for large-scale propagation is hampered also by the low rate of embryo germination; it proves nonetheless very useful for genetic improvement.

Key words

Double regeneration technique Immature and mature zygotic embryos Mature tissues Olea europaea L. var. sativa Olea europaea L. var. sylvestris Somatic embryogenesis 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Agriculture, Forests, Nature and Energy (DAFNE)University of TusciaViterboItaly

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