Abstract
This chapter is designed to present methods for characterizing the annotation of genes associated with mitochondrial functions in Candida spp. Methods include drop plate assays for evaluating inhibitors of the respiratory electron transport system complexes as well as measuring the enzyme activity of complex I-V enzyme activities. Assays are also presented to measure toxic ROS production that accompanies gene mutations or gene loss and chronological aging that often is shortened in Complex I dysfunction. Also presented are methods to isolate mitochondria, visualize mitochondria, and extract mitochondrial proteins.
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Li, D., Calderone, R. (2016). Assessing Mitochondrial Functions in Candida albicans . In: Calderone, R., Cihlar, R. (eds) Candida Species. Methods in Molecular Biology, vol 1356. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3052-4_5
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DOI: https://doi.org/10.1007/978-1-4939-3052-4_5
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3051-7
Online ISBN: 978-1-4939-3052-4
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