Abstract
Interactions of C. albicans with co-colonizing bacteria at mucosal sites can be synergistic or antagonistic in disease development, depending on the bacterial species and mucosal site. Mitis group streptococci and C. albicans colonize the oral mucosa of the majority of healthy individuals. These streptococci have been termed “accessory pathogens,” defined by their ability to initiate multispecies biofilm assembly and promote the virulence of the mixed bacterial biofilm community in which they participate. To demonstrate whether interactions with Mitis group streptococci limit or promote the potential of C. albicans to become an opportunistic pathogen, in vitro and in vivo co-infection models are needed. Here, we describe two C. albicans-streptococcal co-infection models: an organotypic oral mucosal tissue model that incorporates salivary flow and a mouse model of oral co-infection that requires reduced levels of immunosuppression compared to single fungal infection.
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References
Villar CC, Dongari-Bagtzoglou A (2008) Immunodefence mechanisms and immunoenhancement strategies in oropharyngeal candidiasis. Expert Rev Mol Med 10, e29
Dongari-Bagtzoglou A, Kashleva H, Dwivedi P et al (2009) Characterization of mucosal Candida albicans biofilms. PLoS One 4, e7967
Nett JE, Marchillo K, Spiegel CA et al (2010) Development and validation of an in vivo Candida albicans biofilm denture model. Infect Immun 78:3650–3659
Nobile CJ, Fox EP, Nett JE et al (2012) A recently evolved transcriptional network controls biofilm development in Candida albicans. Cell 148:126–138
Morales DK, Hogan DA (2010) Candida albicans interactions with bacteria in the context of human health and disease. PLoS Pathog 6, e1000886
Bamford CV, d’Mello A, Nobbs AH et al (2009) Streptococcus gordonii modulates Candida albicans biofilm formation through intergeneric communication. Infect Immun 77:3696–3704
Silverman RJ, Nobbs AH, Vickerman MM et al (2010) Interaction of Candida albicans cell wall Als3 protein with Streptococcus gordonii SspB adhesin promotes development of mixed-species communities. Infect Immun 78:4644–4652
Diaz PI, Xie Z, Sobue T et al (2012) Synergistic interaction between Candida albicans and commensal oral streptococci in a novel in vitro mucosal model. Infect Immun 80:620–632
Ramsey MM, Rumbaugh KP, Whiteley M (2011) Metabolite cross-feeding enhances virulence in a model polymicrobial infection. PLoS Pathog 7, e1002012
Whitmore SE, Lamont RJ (2011) The pathogenic persona of community-associated oral streptococci. Mol Microbiol 81:305–314
Xu H, Sobue T, Thompson A et al (2013) Streptococcal co-infection augments Candida pathogenicity by amplifying the mucosal inflammatory response. Cell Microbiol 16:214–231
Gillum AM, Tsay EY, Kirsch DR (1984) Isolation of the Candida albicans gene for orotidine-5′-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations. Mol Gen Genet 198:179–182
Dongari-Bagtzoglou A, Kashleva H (2006) Development of a highly reproducible three-dimensional organotypic model of the oral mucosa. Nat Protoc 1:2012–2018
Kalabis J, Wong GS, Vega ME et al (2012) Isolation and characterization of mouse and human esophageal epithelial cells in 3D organotypic culture. Nat Protoc 7:235–246
Xie Z, Thompson A, Kashleva H et al (2011) A quantitative real-time RT-PCR assay for mature C. albicans biofilms. BMC Microbiol 11:93
Dwivedi P, Thompson A, Xie Z et al (2011) Role of Bcr1-activated genes Hwp1 and Hyr1 in Candida albicans oral mucosal biofilms and neutrophil evasion. PLoS One 6, e16218
Acknowledgements
This work was supported by NIH/NIDCR grant RO1 DE013986.
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Sobue, T., Diaz, P., Xu, H., Bertolini, M., Dongari-Bagtzoglou, A. (2016). Experimental Models of C. albicans-Streptococcal Co-infection. In: Calderone, R., Cihlar, R. (eds) Candida Species. Methods in Molecular Biology, vol 1356. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3052-4_10
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DOI: https://doi.org/10.1007/978-1-4939-3052-4_10
Publisher Name: Humana Press, New York, NY
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