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Phospho-Proteomics pp 193–209Cite as

Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview

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Part of the Methods in Molecular Biology book series (MIMB,volume 1355)

Abstract

Magnetic nanocomposites are hybrid structures consisting of an iron oxide (Fe3O4/γ-Fe2O3) superparamagnetic core and a coating shell which presents affinity for a specific target molecule. Within the scope of phosphopeptide enrichment, the magnetic core is usually first functionalized with an intermediate layer of silica or carbon to improve dispersibility and increase specific area, and then with an outer layer of a phosphate-affinity material. Fe3O4-coating materials include metal oxides, rare earth metal-based compounds, immobilized-metal ions, polymers, and many others. This chapter provides a generic overview of the different materials that can be found in literature and their advantages and drawbacks.

Key words

  • Magnetic nanocomposite s
  • Phosphopeptide enrich ment
  • Affinity
  • Hybrid material s

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Acknowledgments

The authors thank the financial support from Fundação para a Ciência e a Tecnologia, Ministério da Educação e da Ciência, Portugal, through projects no. Pest-OE/EQB/LA0004/2011, PEst-C/EQB/LA0006/2013, PTDC/EBB-BIO/102163/2008, PTDC/EBBBIO/118317/2010 and SFRH/BD/64427/2009 for I.L.B.

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Authors and Affiliations

  1. UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal

    Íris L. Batalha & Ana Cecília A. Roque

  2. Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK

    Íris L. Batalha

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  1. Íris L. Batalha
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  2. Ana Cecília A. Roque
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Correspondence to Íris L. Batalha .

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  1. University of Copenhagen, Center for Protein Research, Copenhagen Nm, Denmark

    Louise von Stechow

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Batalha, Í.L., Roque, A.C.A. (2016). Phosphopeptide Enrichment Using Various Magnetic Nanocomposites: An Overview. In: von Stechow, L. (eds) Phospho-Proteomics. Methods in Molecular Biology, vol 1355. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3049-4_13

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  • DOI: https://doi.org/10.1007/978-1-4939-3049-4_13

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