Clickable Polymeric Coating for Oriented Peptide Immobilization
A new methodology for the fabrication of an high-performance peptide microarray is reported, combining the higher sensitivity of a layered Si–SiO2 substrate with the oriented immobilization of peptides using a N,N-dimethylacrylamide-based polymeric coating that contains alkyne monomers as functional groups. This clickable polymer allows the oriented attachment of azido-modified peptides via a copper-mediated azide/alkyne cycloaddition. A similar coating that does not contain the alkyne functionality has been used as comparison, to demonstrate the importance of a proper orientation for facilitating the probe recognition and interaction with the target antibody.
Key wordsPeptide microarray Click chemistry Oriented immobilization Polymeric coating
- 8.Zilio C, Bernardi A, Palmioli A, Salina M, Tagliabue G, Buscaglia M, Consonni R, Chiari M (2015) New “clickable” polymeric coating for glycan microarrays. Sensor Actuator B 215: 412–420Google Scholar