Abstract
Recombinant baculo viruses based on Autographa californica multiple nuclear polyhedrosis virus carrying vertebrate cell active expression cassettes, so-called BacMam viruses, are increasingly used as gene delivery vectors for vaccination of animals against pathogens. Different approaches for generation of BacMams exist and a variety of transfer vectors to improve target protein expression in vivo have been constructed. Here we describe a use of transfer vector which contains an insect cell-restricted expression cassette for the green fluorescent protein and thus enables easy monitoring of BacMam virus rescue, fast plaque purification of recombinants and their convenient titer determination and which has been proven to be efficacious for gene delivery in vaccination/challenge experiments.
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Acknowledgements
This work was supported by the EU Network of Excellence, EPIZONE (Contract No. FOOD-CT-2006-016236) and European Research Project ASFORCE (‘Targeted research effort on African swine fever’, grant agreement 311931) and IDT-Biologika.
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Keil, G.M., Pollin, R., Müller, C., Giesow, K., Schirrmeier, H. (2016). BacMam Platform for Vaccine Antigen Delivery. In: Brun, A. (eds) Vaccine Technologies for Veterinary Viral Diseases. Methods in Molecular Biology, vol 1349. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3008-1_7
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DOI: https://doi.org/10.1007/978-1-4939-3008-1_7
Publisher Name: Humana Press, New York, NY
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