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Peptide Antibodies

Volume 1348 of the series Methods in Molecular Biology pp 23-32

Prediction of Antibody Epitopes

  • Morten NielsenAffiliated withDepartment of Systems Biology, Center for Biological Sequence Analysis, Technical University of DenmarkInstituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín
  • , Paolo MarcatiliAffiliated withDepartment of Systems Biology, Center for Biological Sequence Analysis, Technical University of Denmark Email author 

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Abstract

Antibodies recognize their cognate antigens in a precise and effective way. In order to do so, they target regions of the antigenic molecules that have specific features such as large exposed areas, presence of charged or polar atoms, specific secondary structure elements, and lack of similarity to self-proteins. Given the sequence or the structure of a protein of interest, several methods exploit such features to predict the residues that are more likely to be recognized by an immunoglobulin.

Here, we present two methods (BepiPred and DiscoTope) to predict linear and discontinuous antibody epitopes from the sequence and/or the three-dimensional structure of a target protein.

Keywords

Epitope Linear epitope Discontinuous epitope Prediction