Multiplexed Peptide-MHC Tetramer Staining with Mass Cytometry

  • Mei Ling Leong
  • Evan W. Newell
Part of the Methods in Molecular Biology book series (MIMB, volume 1346)


Mass cytometry is flow cytometry based on single cell mass spectrometry with decreased crosstalk between channels and an ability to probe >40 parameters per cell, making it well suited for multiplexed assays. Peptide major histocompatibility (MHC) tetramer staining allows direct detection of antigen specific cells and is also amenable to multiplexing/combinatorial approaches. Here we describe methods for multiplexed pMHC-tetramer staining using mass cytometry.

Key words

Mass cytometry Peptide-MHC tetramer Antigen-specific T cell Combinatorial tetramer staining 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.School of Biological SciencesNanyang Technological UniversitySingaporeSingapore
  2. 2.Singapore Immunology Network (SIgN), Agency for Science Technology and Research (A-STAR)SingaporeSingapore

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