Abstract
Cellular heterogeneity occurs, and should be probed, at multiple levels of cellular structure and physiology from the genome to enzyme activity. In particular, single-cell measures of protein levels are complemented by single-cell measurements of the activity of these proteins. Microfluidic assays of enzyme activity at the single-cell level combine moderate to high throughput with low dead volumes and the potential for automation. Herein, we describe the steps required to fabricate and operate a microfluidic device for chemical cytometry of fluorescent or fluorogenic reporters of enzyme activity in individual cells.
Key words
- Microfluidic
- Enzyme assay
- Chemical cytometry
- Microchannel
- Reporter substrate
- Electrophoresis
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The authors thank Trinity College for financial support.
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Shehaj, L., de la Vega, L.L., Kovarik, M.L. (2015). Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells. In: Singh, A., Chandrasekaran, A. (eds) Single Cell Protein Analysis. Methods in Molecular Biology, vol 1346. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2987-0_15
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DOI: https://doi.org/10.1007/978-1-4939-2987-0_15
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2986-3
Online ISBN: 978-1-4939-2987-0
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