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Protein Amyloid Aggregation pp 89–100Cite as

Deep UV Resonance Raman Spectroscopy for Characterizing Amyloid Aggregation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1345))

Abstract

Deep UV resonance Raman spectroscopy is a powerful technique for probing the structure and formation mechanism of protein fibrils, which are traditionally difficult to study with other techniques owing to their low solubility and noncrystalline arrangement. Utilizing a tunable deep UV Raman system allows for selective enhancement of different chromophores in protein fibrils, which provides detailed information on different aspects of the fibrils’ structure and formation. Additional information can be extracted with the use of advanced data treatment such as chemometrics and 2D correlation spectroscopy. In this chapter we give an overview of several techniques for utilizing deep UV resonance Raman spectroscopy to study the structure and mechanism of formation of protein fibrils. Clever use of hydrogen-deuterium exchange can elucidate the structure of the fibril core. Selective enhancement of aromatic amino acid side chains provides information about the local environment and protein tertiary structure. The mechanism of protein fibril formation can be investigated with kinetic experiments and advanced chemometrics.

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Acknowledgments

This work was supported by the National Science Foundation under Grant No. CHE-1152752 (IKL).

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Authors and Affiliations

  1. Department of Chemistry, University at Albany, SUNY, 1400 Washington Avenue, Albany, NY, 12222, USA

    Joseph D. Handen & Igor K. Lednev

Authors
  1. Joseph D. Handen
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  2. Igor K. Lednev
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Corresponding author

Correspondence to Igor K. Lednev .

Editor information

Editors and Affiliations

  1. Weill Cornell Medical College, New York, New York, USA

    David Eliezer

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Handen, J.D., Lednev, I.K. (2016). Deep UV Resonance Raman Spectroscopy for Characterizing Amyloid Aggregation. In: Eliezer, D. (eds) Protein Amyloid Aggregation. Methods in Molecular Biology, vol 1345. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2978-8_6

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  • DOI: https://doi.org/10.1007/978-1-4939-2978-8_6

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2977-1

  • Online ISBN: 978-1-4939-2978-8

  • eBook Packages: Springer Protocols

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