Abstract
Cell synchronization is a powerful technique for studying the eukaryotic cell cycle events precisely. The fission yeast is a rod-shaped cell whose growth is coordinated with the cell cycle. Monitoring the cellular growth of fission yeast is a relatively simple way to measure the cell cycle stage of a cell. Here, we describe a detailed method of unperturbed cell synchronization, named centrifugal elutriation, for fission yeast.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Mitchison JM (2003) Growth during the cell cycle. Int Rev Cytol 226:165–258
Humphery T, Brooks G (2005) Cell cycle control. In: Humphery T, Brooks G (eds) Methods in molecular biology, vol 296. Humana Press, Totowa, NJ, pp 1–29
Hayles J, Nurse P (2001) A journey into space. Nat Rev Mol Cell Biol 2:647–656
Martin SG, Chang F (2005) New end take off: regulating cell polarity during the fission yeast cell cycle. Cell Cycle 4:4046–4049
Navarro FJ, Weston L, Nurse P (2012) Global control of cell growth in fission yeast and its coordination with the cell cycle. Curr Opin Cell Biol 24:1–5
Walker GM (1999) Synchronization of yeast cell populations. Methods Cell Sci 21:87–93
Forsburg SL, Rhind N (2006) Basic methods for fission yeast. Yeast 23:173–183
Kume K, Koyano T, Kanai M, Toda T, Hirata D (2011) Calcineurin ensures a link between the DNA replication checkpoint and microtubule-dependent polarized growth. Nat Cell Biol 13:234–242
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Kume, K. (2016). Elutriation for Cell Cycle Synchronization in Fission Yeast. In: Coutts, A., Weston, L. (eds) Cell Cycle Oscillators. Methods in Molecular Biology, vol 1342. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2957-3_7
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2957-3_7
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2956-6
Online ISBN: 978-1-4939-2957-3
eBook Packages: Springer Protocols