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Elutriation for Cell Cycle Synchronization in Fission Yeast

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Cell Cycle Oscillators

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1342))

Abstract

Cell synchronization is a powerful technique for studying the eukaryotic cell cycle events precisely. The fission yeast is a rod-shaped cell whose growth is coordinated with the cell cycle. Monitoring the cellular growth of fission yeast is a relatively simple way to measure the cell cycle stage of a cell. Here, we describe a detailed method of unperturbed cell synchronization, named centrifugal elutriation, for fission yeast.

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Correspondence to Kazunori Kume .

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Kume, K. (2016). Elutriation for Cell Cycle Synchronization in Fission Yeast. In: Coutts, A., Weston, L. (eds) Cell Cycle Oscillators. Methods in Molecular Biology, vol 1342. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2957-3_7

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  • DOI: https://doi.org/10.1007/978-1-4939-2957-3_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2956-6

  • Online ISBN: 978-1-4939-2957-3

  • eBook Packages: Springer Protocols

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