Abstract
Quantitative measurement of enzyme activity is a valuable approach to study how cells function. We present a method to measure the activity of the enzyme Cdk1/cyclin B. This enzyme is required by all eukaryotic cells to enter mitosis. Therefore, a biochemical assay to measure Cdk1/cyclin B activity can be used to identify cell populations that are in mitosis or to detect inhibitors of Cdk1/Cyclin B in vitro. A key distinction of the method presented here, compared to others, is that it uses a recombinant protein, a specific antibody, and a western blot apparatus, which makes the technique available to cell and molecular biology laboratories who do not wish to use radioisotopes, which are commonly required for other protein kinase assays.
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Lewis, C.W., Taylor, R.G., Golsteyn, R.M. (2016). Measurement of Cdk1/Cyclin B Kinase Activity by Specific Antibodies and Western Blotting. In: Coutts, A., Weston, L. (eds) Cell Cycle Oscillators. Methods in Molecular Biology, vol 1342. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2957-3_21
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DOI: https://doi.org/10.1007/978-1-4939-2957-3_21
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2956-6
Online ISBN: 978-1-4939-2957-3
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