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Mesenchymal Stem Cells Derived from Human Adipose Tissue

  • Protocol
Book cover Cartilage Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1340))

Abstract

Human adult mesenchymal stem cells are present in fat tissue, which can be obtained using surgical procedures such as liposuction. The multilineage capacity of mesenchymal stem cells makes them very valuable for cell-based medical therapies. In this chapter, we describe how to isolate mesenchymal stem cells from human adult fat tissue, propagate the cells in culture, and cryopreserve the cells for tissue engineering applications. Flow cytometry methods are also described for identification and characterization of adipose-derived stem cells and for cell sorting.

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Author information

Authors and Affiliations

  1. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia

    Nastaran Mahmoudifar

  2. Faculty of Science, Engineering and Technology, Swinburne University of Technology, P.O. Box 218, Hawthorn, Melbourne, VIC, 3122, Australia

    Pauline M. Doran

Authors
  1. Nastaran Mahmoudifar
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  2. Pauline M. Doran
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Corresponding author

Correspondence to Pauline M. Doran .

Editor information

Editors and Affiliations

  1. Swinburne University of Technology, Faculty of Science, Engineering and Technology, Hawthorn, Melbourne, Victoria, Australia

    Pauline M. Doran

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© 2015 Springer Science+Business Media New York

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Mahmoudifar, N., Doran, P.M. (2015). Mesenchymal Stem Cells Derived from Human Adipose Tissue. In: Doran, P. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 1340. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2938-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-2938-2_4

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2937-5

  • Online ISBN: 978-1-4939-2938-2

  • eBook Packages: Springer Protocols

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