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Human Fetal and Adult Chondrocytes

  • Protocol
Cartilage Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1340))

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Abstract

As the only cell type found in healthy adult cartilage, chondrocytes are the obvious and most direct starting point for cartilage tissue engineering. Human adult, juvenile, neonatal, and fetal chondrocytes have all been demonstrated to produce cartilage matrix components in vitro for production of engineered tissues. In this chapter, procedures are outlined for isolation of chondrocytes from human fetal and adult cartilage. Methods for expansion and cryopreservation of the cells and characterization of gene expression using quantitative polymerase chain reaction (Q-PCR) analysis are also described.

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Author information

Authors and Affiliations

  1. Westmead Millennium Institute for Medical Research, University of Sydney, Westmead, NSW, 2145, Australia

    Kifah Shahin

  2. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia

    Nastaran Mahmoudifar

  3. Faculty of Science, Engineering and Technology, Swinburne University of Technology, P.O. Box 218, Hawthorn, Melbourne, VIC, 3122, Australia

    Pauline M. Doran

Authors
  1. Kifah Shahin
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  2. Nastaran Mahmoudifar
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  3. Pauline M. Doran
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Corresponding author

Correspondence to Kifah Shahin .

Editor information

Editors and Affiliations

  1. Swinburne University of Technology, Faculty of Science, Engineering and Technology, Hawthorn, Melbourne, Victoria, Australia

    Pauline M. Doran

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Shahin, K., Mahmoudifar, N., Doran, P.M. (2015). Human Fetal and Adult Chondrocytes. In: Doran, P. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 1340. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2938-2_2

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  • DOI: https://doi.org/10.1007/978-1-4939-2938-2_2

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2937-5

  • Online ISBN: 978-1-4939-2938-2

  • eBook Packages: Springer Protocols

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