Abstract
Mechanical forces, including hydrodynamic shear, hydrostatic pressure, compression, tension, and friction, can have stimulatory effects on cartilage synthesis in tissue engineering systems. Bioreactors capable of exerting forces on cells and tissue constructs within a controlled culture environment are needed to provide appropriate mechanical stimuli. In this chapter, we describe the construction, assembly, and operation of a mechanobioreactor providing simultaneous dynamic shear and compressive loading on developing cartilage tissues to mimic the rolling and squeezing action of articular joints. The device is suitable for studying the effects of mechanical treatment on stem cells and chondrocytes seeded into three-dimensional scaffolds.
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Acknowledgements
This work was funded by the Australian Research Council (ARC). We are grateful to Russell Cail for assistance with design of the mechanobioreactor and John Matiossa for device fabrication and workshop services.
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Shahin, K., Doran, P.M. (2015). Shear and Compression Bioreactor for Cartilage Synthesis. In: Doran, P. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 1340. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2938-2_16
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DOI: https://doi.org/10.1007/978-1-4939-2938-2_16
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2937-5
Online ISBN: 978-1-4939-2938-2
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