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Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays

  • Junling Wang
  • Anna S. KashinaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1337)

Abstract

Here we describe the procedure for expression and purification of recombinant ATE1 from E. coli. This method is easy and convenient and can result in one-step isolation of milligram amounts of soluble enzymatically active ATE1 at nearly 99 % purity. We also describe a procedure for expression and purification of E. coli Arg-tRNA synthetase essential for the arginylation assays described in the next two chapters.

Key words

Arginyltransferase Arg-tRNA synthetase Recombinant protein Arginylation 

References

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Animal BiologyUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Department of Animal Biology, School of Veterinary MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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