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Correlated Measurement of Endogenous ATE1 Activity on Native Acceptor Proteins in Tissues and Cultured Cells to Detect Cellular Aging

  • Hideko KajiEmail author
  • Akira KajiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1337)

Abstract

Following our early discovery of arginylation in 1963, we have performed several studies to correlate its activity with essential biological processes. We employed cell- and tissue-based assays to detect both the level of acceptor proteins and the level of ATE1 activity under different conditions. Remarkably, in these assays, we found a close correlation between arginylation and aging, a discovery that we believe has longer-term implications in uncovering the importance of ATE1 in normal biology and disease therapies. Here we describe the original methods we used to measure ATE1 activity in tissues and correlate it with key biological events.

Key words

Arginylation Aging Cell proliferation SV40 transformation ATE1 Arg acceptor proteins 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular Biology, Jefferson Medical CollegeThomas Jefferson UniversityPhiladelphiaUSA
  2. 2.Department of Microbiology, Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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