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Preparation of ATE1 Enzyme from Native Mammalian Tissues

  • Anna S. KashinaEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 1337)

Abstract

Early studies of protein arginylation preceded the wide use of recombinant protein expression and relied heavily on fractionation of proteins from native tissues. The procedure described below has been developed in 1970 by R. Soffer, in the wake of arginylation discovery in 1963. This chapter follows the detailed procedure originally published by R. Soffer in the 1970, adapted from his article in consultation with R. Soffer, H. Kaji, and A. Kaji.

Key words

Arginyltransferase Native enzyme purification Arginylation 

References

  1. 1.
    Soffer RL (1970) Enzymatic modification of proteins. II. Purification and properties of the arginyl transfer ribonucleic acid-protein transferase from rabbit liver cytoplasm. J Biol Chem 245(4):731–737PubMedGoogle Scholar
  2. 2.
    Kaji H, Novelli GD, Kaji A (1963) A soluble amino acid-incorporating system from rat liver. Biochim Biophys Acta 76:474–477CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Animal Biology School of Veterinary MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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