Recollection of How We Came Across the Protein Modification with Amino Acids by Aminoacyl tRNA-Protein Transferase

  • Hideko KajiEmail author
  • Akira KajiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1337)


Protein arginylation has been discovered in 1963 as a soluble activity in cell extracts that mediates addition of amino acids to proteins. This discovery was nearly accidental, but due to the persistence of the research team, it has been followed through and led to the emergence of a new field of research. This chapter describes the original discovery of arginylation and the first methods used to demonstrate the existence of this important biological process.

Key words

Discovery of arginylation Soluble amino acid-incorporating system Assaying arginylation in cell extracts 


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular Biology, Jefferson Medical CollegeThomas Jefferson UniversityPhiladelphiaUSA
  2. 2.Department of Microbiology, Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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