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Analysis of Arginylated Peptides by Subtractive Edman Degradation

  • Anna S. Kashina
  • John R. YatesIIIEmail author
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Part of the Methods in Molecular Biology book series (MIMB, volume 1337)

Abstract

During the early studies of N-terminal arginylation, Edman degradation was widely used to identify N-terminally added Arg on protein substrates. This old method is reliable but highly depends on the purity and abundance of samples and can become misleading unless a highly purified, highly arginylated protein can be obtained. Here we report a mass spectrometry-based method that utilizes Edman degradation chemistry to identify arginylation in more complex and less abundant protein samples. This method can also apply to the analysis of other posttranslational modifications.

Key words

Edman degradation Mass spectrometry Arginylation 

References

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    Wang J, Han X, Wong CC, Cheng H, Aslanian A, Xu T, Leavis P, Roder H, Hedstrom L, Yates JR III, Kashina A (2014) Arginyltransferase ATE1 catalyzes midchain arginylation of proteins at side chain carboxylates in vivo. Chem Biol 21(3):331–337PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Animal Biology, School of Veterinary MedicineUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Department of Chemical PhysiologyThe Scripps Research InstituteLa JollaUSA

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