Abstract
Immunostaining can be defined as a method for identification of constituents in situ, e.g., proteins, using specific antigen–antibody interactions, visualized by color or fluorescence. In experimental research of atherosclerosis, immunostaining is a key method used for the characterization of the cellular composition in the atherosclerotic plaques. This includes the quantification of macrophages, smooth muscle cells, and lymphocytes—especially T lymphocytes. In this chapter we focus on basic protocols for characterization of lymphocytes and/or local T lymphocyte-related antigens in mouse atherosclerotic lesions.
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Acknowledgements
We gratefully acknowledge Inger Bodin and Linda Haglund for their technical assistant, standardization, and optimization of immunostaining methods at our Unit. We thank Konstantinos Polyzos for preparation of representative micrographs. We thank Antonino Nicoletti, Xinghua Zhou, Anna-Karin Robertson, and Inger Bodin for the implementation of the laboratory’s sectioning protocol.
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Gisterå, A., Ketelhuth, D.F.J. (2015). Immunostaining of Lymphocytes in Mouse Atherosclerotic Plaque. In: Andrés, V., Dorado, B. (eds) Methods in Mouse Atherosclerosis. Methods in Molecular Biology, vol 1339. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2929-0_10
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DOI: https://doi.org/10.1007/978-1-4939-2929-0_10
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