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The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens

  • Protocol
Carbohydrate-Based Vaccines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1331))

Abstract

Carbohydrate antigens are important targets for the immune system, but identification of key glycan antigens is challenging. Direct analysis of glycomes by mass spectrometry is difficult, and detection reagents, such as monoclonal antibodies and lectins, are only available for a small subset of glycans. An alternative approach involves profiling serum anti-glycan antibody populations to identify unique antibodies or changes in antibody subpopulations. Glycan microarray technology allows rapid evaluation of hundreds to thousands of antigen-antibody interactions in a single experiment. This high-throughput format is particularly useful in profiling complex anti-glycan antibodies in serum. Here we elaborate the use of this technology to explore clinically relevant carbohydrate antigens by profiling serum anti-glycan antibodies. Detailed protocols from glycan microarray fabrication to microarray binding assays and analysis of microarray data are presented.

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Acknowledgement

This work was supported by Intramural Research Program in National Institutes of Health.

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Authors and Affiliations

  1. Chemical Biology Laboratory, National Cancer Institute, 376 Boyles Street, Building 376, Frederick, MD, 21702, USA

    Li Xia & Jeffrey C. Gildersleeve

Authors
  1. Li Xia
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  2. Jeffrey C. Gildersleeve
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Corresponding author

Correspondence to Jeffrey C. Gildersleeve .

Editor information

Editors and Affiliations

  1. Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine, Hannover, Germany

    Bernd Lepenies

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Xia, L., Gildersleeve, J.C. (2015). The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens. In: Lepenies, B. (eds) Carbohydrate-Based Vaccines. Methods in Molecular Biology, vol 1331. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2874-3_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2874-3_3

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2873-6

  • Online ISBN: 978-1-4939-2874-3

  • eBook Packages: Springer Protocols

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