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Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial β-Barrel Proteins

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The BAM Complex

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1329))

Abstract

Beta-barrel proteins are found in the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts. The evolutionary conservation in the biogenesis of these proteins allows mitochondria to assemble bacterial β-barrel proteins in their functional form. In this chapter, we describe exemplarily how the capacity of yeast mitochondria to process the trimeric autotransporter YadA can be used to study the role of bacterial periplasmic chaperones in this process.

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Acknowledgments

Our work is supported by the Deutsche Forschungsgemeinschaft (SFB766/TP B11 and RA 1028/7-1 to D.R. and SFB766/TP B1 to I.A.) and by the UKT fortüne program (F1433253 to P.O.).

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Authors and Affiliations

  1. Interfaculty Institute of Biochemistry, University of Tübingen, Hoppe-Seyler-Straße 4, Tübingen, 72076, Germany

    Thomas Ulrich & Doron Rapaport

  2. Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, 72076, Germany

    Philipp Oberhettinger & Ingo B. Autenrieth

Authors
  1. Thomas Ulrich
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  2. Philipp Oberhettinger
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  3. Ingo B. Autenrieth
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  4. Doron Rapaport
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Corresponding author

Correspondence to Doron Rapaport .

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Editors and Affiliations

  1. National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Laboratory of Molecular Biology, , Bethesda, Maryland, USA

    Susan Buchanan

  2. Department of Biological Sciences,, Markey Center for Structural Biology, Purdue University, West Lafayette, Indiana, USA

    Nicholas Noinaj

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Ulrich, T., Oberhettinger, P., Autenrieth, I.B., Rapaport, D. (2015). Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial β-Barrel Proteins. In: Buchanan, S., Noinaj, N. (eds) The BAM Complex. Methods in Molecular Biology, vol 1329. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2871-2_2

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  • DOI: https://doi.org/10.1007/978-1-4939-2871-2_2

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2870-5

  • Online ISBN: 978-1-4939-2871-2

  • eBook Packages: Springer Protocols

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