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Muscarinic Receptor: From Structure to Animal Models pp 159–180Cite as

Autoradiography Assessment of Muscarinic Receptors in the Central Nervous System

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Part of the book series: Neuromethods ((NM,volume 107))

Abstract

The detection of muscarinic receptor binding sites is a crucial step in many experimental conditions. Although in peripheral tissue, the radioligand binding (see appropriate chapter) allows to obtain well-defined receptor characteristic, and also is usable in some central nervous system regions, when trying to determine receptor binding in central nervous system regions with low density or with infinitesimally small receptor changes, the receptor autoradiography is a better method. The development of this method made important progress, and some different modes (phosphor imaging) are used nowadays. Here, we describe muscarinic receptor detection using different radioligands: [3H]-QNB, [3H]-NMS, [3H]-pirenzepine, and [3H]-AFDX-384. Specific attention is paid to the detection of subtypes of muscarinic receptors and the limits of the method are emphasized.

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Abbreviations

3H-QNB:

3H-quinuclidinyl benzilate, 3H-1-azabicyclo[2.2.2]oct-3-yl 2-hydroxy-2, 2-diphenylacetate

3H-NMS:

3H-N-methyl-scopolamine, 3H-(1R,2S,4R,5S,7R)-{[(2R)-3-hydroxy-2-phenylpropanoyl]oxy}s[19]-9,9-dimethyl-3-oxa-9-azoniatricyclo [3.3.1.02,4]nonane

3H-pirenzepine:

3H-11-[(4-methylpiperazin-1-yl)acetyl]-5,11-dihydro-6H-pyrido[2,3-b][1,4]benzodiazepin-6-one

3H-AFDX-384:

3H-N-(2-[(2R)-2-[(dipropylamino)methyl]piperidin-1-yl]ethyl)-6-oxo-5H-pyrido[2,3-b][1,4]benzodiazepine-11-carboxamide

RT:

Room temperature

MRs:

Muscarinic receptors

GPCRs:

G protein-coupled receptors

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Acknowledgments

The research on this topic was supported by grant GAUK 328314 from Grant Agency of Charles University and by projects PRVOUK P25/1LF and PRVOUK P35/1LF.

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Authors and Affiliations

  1. Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University, 12800, Prague, Czech Republic

    Vladimir Farar

  2. Institute of Physiology, 1st Faculty of Medicine, Charles University, Albertov 5, 12800, Prague, Czech Republic

    Jaromir Myslivecek

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  1. Vladimir Farar
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Correspondence to Jaromir Myslivecek .

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Editors and Affiliations

  1. Institute of Physiology, Charles University, Prague, Czech Republic

    Jaromir Myslivecek

  2. Department of Neurochemistry, Acad. of Sciences of the Czech Rep. Institute of Physiology, Prague, Czech Republic

    Jan Jakubik

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Farar, V., Myslivecek, J. (2016). Autoradiography Assessment of Muscarinic Receptors in the Central Nervous System. In: Myslivecek, J., Jakubik, J. (eds) Muscarinic Receptor: From Structure to Animal Models. Neuromethods, vol 107. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2858-3_9

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  • DOI: https://doi.org/10.1007/978-1-4939-2858-3_9

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2857-6

  • Online ISBN: 978-1-4939-2858-3

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