Abstract
A small subpopulation of non-replicating, multidrug-tolerant bacteria is present within clonal populations of many bacterial species. Known as persisters, these bacteria are probably the cause of relapsing infections such as typhoid fever. Formation of non-growing Salmonella persisters is stimulated by macrophage phagocytosis. This chapter outlines methods to identify and study persisters resulting from interactions between bacterial pathogens and their hosts. We use their antibiotic tolerance for isolation and enumeration and developed a method to study the heterogeneity of growth within clonal populations through single-cell analysis.
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References
Helaine S, Cheverton AM, Watson KG, Faure LM, Matthews SA, Holden DW (2014) Internalization of Salmonella by macrophages induces formation of nonreplicating persisters. Science 343(6167):204–208. doi:10.1126/science.1244705
Helaine S, Thompson JA, Watson KG, Liu M, Boyle C, Holden DW (2010) Dynamics of intracellular bacterial replication at the single cell level. Proc Natl Acad Sci U S A 107(8):3746–3751. doi:10.1073/pnas.1000041107
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Fisher, R.A., Cheverton, A.M., Helaine, S. (2016). Analysis of Macrophage-Induced Salmonella Persisters. In: Michiels, J., Fauvart, M. (eds) Bacterial Persistence. Methods in Molecular Biology, vol 1333. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2854-5_15
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DOI: https://doi.org/10.1007/978-1-4939-2854-5_15
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2853-8
Online ISBN: 978-1-4939-2854-5
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