Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species

  • Inbar Friedrich Ben-Nun
  • Susanne C. Montague
  • Marlys L. Houck
  • Oliver Ryder
  • Jeanne F. Loring
Part of the Methods in Molecular Biology book series (MIMB, volume 1330)


For some highly endangered species there are too few reproductively capable animals to maintain adequate genetic diversity, and extraordinary measures are necessary to prevent their extinction. Cellular reprogramming is a means to capture the genomes of individual animals as induced pluripotent stem cells (iPSCs), which may eventually facilitate reintroduction of genetic material into breeding populations. Here, we describe a method for generating iPSCs from fibroblasts of mammalian endangered species.

Key words

Induced pluripotent stem cells Cellular reprogramming Endangered species Conservation 



This work was supported by the Esther O’Keefe Foundation and the Millipore Foundation. S.M. was supported by a Bridges to Stem Cell Research grant from the California Institute for Regenerative Medicine (TB1-01186).


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Inbar Friedrich Ben-Nun
    • 1
  • Susanne C. Montague
    • 1
  • Marlys L. Houck
    • 2
  • Oliver Ryder
    • 2
  • Jeanne F. Loring
    • 1
    • 3
  1. 1.Department of Chemical Physiology, Center for Regenerative MedicineThe Scripps Research InstituteLa JollaUSA
  2. 2.San Diego Zoo Institute for Conservation ResearchEscondidoUSA
  3. 3.Department of Reproductive MedicineUniversity of California, San DiegoLa JollaUSA

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