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An Imaging System for C. elegans Behavior

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C. elegans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1327))

Abstract

Many experiments in C. elegans neurobiology and development benefit from automated imaging of worm behavior. Here we describe procedures for building a flexible and inexpensive imaging system using standard optical and mechanical components.

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References

  1. White J, Southgate E, Thomson J, Brenner S (1986) The structure of the nervous system of the nematode Caenorhabditis elegans. Philos Trans R Soc Lond B 314:1–340. doi:10.1098/rstb.1986.0056

    Article  CAS  Google Scholar 

  2. Richmond J (2005) Synaptic function. WormBook Online Rev C Elegans Biol 1–14. doi: 10.1895/wormbook.1.69.1

  3. Goodman MB (2006) Mechanosensation. WormBook Online Rev C Elegans Biol 1–14. doi: 10.1895/wormbook.1.62.1

  4. Bargmann CI (2006) Chemosensation in C. elegans. WormBook Online Rev C Elegans Biol 1–29. doi:10.1895/wormbook.1.123.1

  5. Von Stetina SE, Treinin M, Miller DM (2006) The motor circuit. Int Rev Neurobiol 69:125–167. doi:10.1016/S0074-7742(05)69005-8

    Article  Google Scholar 

  6. Avery L, You Y-J (2012) C. elegans feeding. WormBook Online Rev C Elegans Biol 1–23. doi: 10.1895/wormbook.1.150.1

  7. Hart A (2006) Behavior. WormBook. http://www.wormbook.org/chapters/www_behavior/behavior.html. Accessed 1 Aug 2012

  8. Husson SJ, Costa WS, Schmitt C, Gottschalk A (2012) Keeping track of worm trackers. WormBook Online Rev C Elegans Biol 1–17. doi: 10.1895/wormbook.1.156.1

  9. Yu C-CJ, Raizen DM, Fang-Yen C (2014) Multi-well imaging of development and behavior in Caenorhabditis elegans. J Neurosci Methods 223:35–39. doi:10.1016/j.jneumeth.2013.11.026

    Article  PubMed Central  PubMed  Google Scholar 

  10. Churgin MA, Yu CC, Chen X, Raizen DM, Fang-Yen C (unpublished manuscript). High-throughput longitudinal monitoring of C. elegans healthspan and longevity using a multi-well device

    Google Scholar 

  11. Clark DA, Gabel CV, Lee TM, Samuel ADT (2007) Short-term adaptation and temporal processing in the cryophilic response of Caenorhabditis elegans. J Neurophysiol 97:1903–1910. doi:10.1152/jn.00892.2006

    Article  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Bioengineering, University of Pennsylvania, Skirkanich Hall 240, 210 S. 33rd Street, Philadelphia, PA, 19104, USA

    Matthew A. Churgin & Christopher Fang-Yen

  2. Department of Neuroscience, University of Pennsylvania, Skirkanich Hall 240, 210 S. 33rd Street, Philadelphia, PA, 19104, USA

    Christopher Fang-Yen

Authors
  1. Matthew A. Churgin
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  2. Christopher Fang-Yen
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Corresponding author

Correspondence to Christopher Fang-Yen .

Editor information

Editors and Affiliations

  1. Department of Physics, The James Franck Institution, The Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA

    David Biron

  2. Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, New Jersey, USA

    Gal Haspel

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© 2015 Springer Science+Business Media New York

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Churgin, M.A., Fang-Yen, C. (2015). An Imaging System for C. elegans Behavior. In: Biron, D., Haspel, G. (eds) C. elegans. Methods in Molecular Biology, vol 1327. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4939-2842-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-2842-2_14

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4939-2841-5

  • Online ISBN: 978-1-4939-2842-2

  • eBook Packages: Springer Protocols

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