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Miniaturized Growth Inhibition Assay to Assess the Anti-blood Stage Activity of Antibodies

  • Elizabeth H. Duncan
  • Elke S. Bergmann-Leitner
Part of the Methods in Molecular Biology book series (MIMB, volume 1325)

Abstract

While no immune correlate for blood-stage specific immunity against Plasmodium falciparum malaria has been identified, there is strong evidence that antibodies directed to various malarial antigens play a crucial role. In an effort to evaluate the role of antibodies in inhibiting growth and/or invasion of erythrocytic stages of the malaria parasite it will be necessary to test large sample sets from Phase 2a/b trials as well as epidemiological studies. The major constraints for such analyses are (1) availability of sufficient sample quantities (especially from infants and small children) and (2) the throughput of standard growth inhibition assays. The method described here assesses growth- and invasion inhibition by measuring the metabolic activity and viability of the parasite (by using a parasite lactate dehydrogenase-specific substrate) in a 384-microtiter plate format. This culture method can be extended beyond the described detection system to accommodate other techniques commonly used for growth/invasion-inhibition.

Key words

Plasmodium Antibodies Growth inhibition High-throughput Functional assay 

Notes

Acknowledgements

The authors would like to thank Dr. Wolfgang Leitner for critical reading of the manuscript.

Disclaimer The opinions or assertions contained herein are the private views of the authors, and are not to be construed as official, or as reflecting true views of the Department of the Army or the Department of Defense.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Elizabeth H. Duncan
    • 1
  • Elke S. Bergmann-Leitner
    • 1
  1. 1.Malaria Vaccine BranchWalter Reed Army Institute of ResearchSilver SpringUSA

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