Abstract
The efficient transfection of cloned genes into cells has a critical role in nucleic acid-based therapeutic applications, molecular and cell biology studies, and the production of recombinant proteins in cultured cells. Using a stearylated cell-penetrating peptide (CPP) NickFect51, we have generated an effective, universal, and convenient method for the delivery of DNA vectors into animal cells derived from different origins (mammalian, avian, insect). The CPP-mediated transfection described in detail herein is efficient for many regular cell lines commonly used for research purposes and it is especially suitable for transfection of protein production cell lines adapted for growth in chemically defined serum-free medium.
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Acknowledgements
We warmly thank Ivar Ilves, Margit Ool, and Mart Toots for their assistance in experimental work. We also thank Anne Kalling, Andres Tover, Urve Toots, and Mart Ustav for their support.
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Karro, K., Männik, T., Männik, A., Ustav, M. (2015). DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent. In: Langel, Ü. (eds) Cell-Penetrating Peptides. Methods in Molecular Biology, vol 1324. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2806-4_29
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DOI: https://doi.org/10.1007/978-1-4939-2806-4_29
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2805-7
Online ISBN: 978-1-4939-2806-4
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